DNA Structure and Formation Lab
 
 
 
 
 

Procedure: I began the bacterial DNA extraction by washing my hands. Next I measured 1.0 ml of sodium dodecyl sulfate into the E. coli culture, using a pipette. Then I placed the cap back on the tube and rolled it between my hands slowly.
 The next step I took, was to place the lyced solution into a pre heated bath, which was 60-65 degrees Celsius for 30 minutes. Then I measured 10 ml of 95% ethanol and removed the lysate from the water bath, allowing it to cool to room temperature. Then I carefully placed the spooling rod into the E. coli suspension and used a pipette to add the cold ethanol to the spooling tube. I tilted the tube at a 45% angle to prevent the layers from mixing. Next I began to rotate the spooling rod clockwise until a mass of DNA was visible. After viewing the DNA I removed the rod and placed it in a 95% ethanol solution.
 During the experiment with DNA and RNA I was able to replicate in simple form DNA, mRNA, and tRNA using a commercial DNA kit.
 

Results: The visible formation of DNA in the bacterial extraction. Was due to the amount of nucleotides, which combined to form a chain inside of the test tube.

1. AAG     CCU     GCG      UUA      GUG
Lysine   Proline  Alanine  Leucine  Histidine

2.   TAC     GCA   CCA  AGA  CGA  CAA
      TAC     GCG   CCG  AGG  CGG  CAG

3. Two genes can code for the same amino acid but they can affect it in different ways.

4. 99
 

5. They pick up and transfer amino acid molecules from the cytoplasm to the mitochondria

6. DNA uses a nucleotide known as Thymine. RNA replaces Thymine with Uricil, which makes it different. DNA is not able to travel outside of the nucleus, and RNA is produced to travel outside of the nucleus to the cytoplasm and its entire mission is to deliver the message from the DNA in the nucleus to a new location safely.
 

Concision: In the process of doing the lab I was able to see for the first time an actual DNA molecule being formed. I was also able to recreate several different combinations of triplet coding. Which hold the keys for amino acids. The simplified puzzle pieces that I used were very helpful in aiding my understanding of the DNA structure.