Mitosis Lab
Materials used: die, micropipette, blood, gel
trey, gel, pipette tips, electric box, water, and latex gloves.
Procedure: The main purpose of this lab was
to gain a first hand experience using the micropipette. And to better understand
meiosis and its affects on the construction of chromosomes and there constituents.
First I put on latex gloves then, I
placed the prepared gel in the gel tray and added enough water to fill
the trey to the rim. The second step I took was placing a plastic tip on
the micropipette. Then I used the micropipette to absorb a small amount
of blood from a prepared tube. Next I released the enclosed blood into
the gel slots (after each use of the micropipette I used a new plastic
tip). The Last step was attaching the electric box to the gel tray and
allowing electric current to flow through the water. After a significant
amount of time passed I recorded my results.
In the second portion of the lab I preformed
the first and second stages of meiosis (on a soon to be mystical unicorn).
The first step was to separate two chromosomes into four chromatids, labeling
the parts of the chromatids. Then I combined the chromatids with four other
chromatids (using a pair of die for variation of matching). Next I combined
my chromosomal combinations with those of other groups, who had gone through
the same chromosomal separations that I had. The final step I took was
placing the genetic material together, to form two different unicorns.
Results: The results of this lab proved that carriers of the sickle cell trait. Display traces of, genetic material that is found both in normal B blood and O blood types.
Conclusion: By performing this lab I was able
to gain a better understanding of how meiosis goes through 2 cycles instead
of 1. I also learned better techniques with the micropipette.