Mitosis Lab



 


 

Materials used: die, micropipette, blood, gel trey, gel, pipette tips, electric box, water, and latex gloves.
 

Procedure: The main purpose of this lab was to gain a first hand experience using the micropipette. And to better understand meiosis and its affects on the construction of chromosomes and there constituents.
 First I put on latex gloves then, I placed the prepared gel in the gel tray and added enough water to fill the trey to the rim. The second step I took was placing a plastic tip on the micropipette. Then I used the micropipette to absorb a small amount of blood from a prepared tube. Next I released the enclosed blood into the gel slots (after each use of the micropipette I used a new plastic tip). The Last step was attaching the electric box to the gel tray and allowing electric current to flow through the water. After a significant amount of time passed I recorded my results.
 In the second portion of the lab I preformed the first and second stages of meiosis (on a soon to be mystical unicorn). The first step was to separate two chromosomes into four chromatids, labeling the parts of the chromatids. Then I combined the chromatids with four other chromatids (using a pair of die for variation of matching). Next I combined my chromosomal combinations with those of other groups, who had gone through the same chromosomal separations that I had. The final step I took was placing the genetic material together, to form two different unicorns.
 

Results: The results of this lab proved that carriers of the sickle cell trait. Display traces of, genetic material that is found both in normal B blood and O blood types.




Conclusion: By performing this lab I was able to gain a better understanding of how meiosis goes through 2 cycles instead of 1. I also learned better techniques with the micropipette.
 
 

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