Who exactly Virgo was considered to represent is uncertain; in history, it has been associated with nearly every prominent goddess, including Ishtar, Isis, Cybele, Mary, Mother of Jesus, and Athena. Virgo may also feature, along with Ursa Major, and Ursa Minor, as part of the source of the myth of Callisto, either as Callisto herself, or as Hera. Persephone (who in some mythologies, notably the Eleusinian Mysteries, was considered to be a form of Demeter) is often mentioned as well, Virgo being visible mainly in the spring months when she was believed to have risen from the underworld.

According to one interpretation, the constellation depicts Astraea, the virgin daughter of the god Zeus and the goddess Themis. Astraea was known as the goddess of justice, and was identified as this constellation due to the presence of the scales of justice Libra nearby, and supposedly ruled the world at one point with her wise ways until mankind became so callous she returned to skies disgusted.