if digesting to check a plasmid on a gel, incubate at 37C for at least one hour. if digesting prior to ligation of an insert, look up restriction enzyme efficiency in a catalogue such as new england biolabs. also, see ligation.

ingredients

For each 20ul reaction, in a 1.5ul microfuge tube:

After incubation in a water bath at 37C for at least 1 hr, add 2ul 10X DNA sample buffer to each and run on a agarose gel. Don't forget your markers!

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