LB 2x YT

LB

5g yeast extraction

10g tryptone

10g NaCl

(18g bacto agar if making plates)

1L H2O

Autoclave. Cool until ok to hold bottom of flask. Add any necessary selective medium.

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2x YT

8g yeast extraction

16g tryptone

5g NaCl

(18g bacto agar if making plates)

1L H2O

Autoclave. Cool until ok to hold bottom of flask. Add any necessary selective medium.

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