By Admin (Admin) on Monday, May 21, 2001 - 01:52 pm: Edit

Materials

1/2 pint wide mouth jar
bottled water
1 tsp honey
saran wrap
rubber band
masking tape
microwave oven
2 cup microwave safe measuring cup
Sterile spore syringe
Disinfectant such as Lysol or bleach water in a spray bottle
Pot holder or oven mitt

Procedure

Have all materials at hand so you can work quickly.
Put about 1 1/8 cups water in the measuring cup.
Add about 1 tsp honey to the water and stir until dissolved.
Place the cup and the half-pint jar in the microwave.
Fry on high for five minutes
Open the microwave do not remove the cup and jar.
Working quickly within the microwave and using the potholder to protect your hand, pour water into the jar to about the bottom of the threads. Stretch saran wrap over the top of the jar and secure with a rubber band.
Let cool to room temperature.
Clean a working surface such as a table with a disinfectant such as Lysol or bleach water.
Mist the surrounding air with the disinfectant.
Working quickly inoculate the jar through the saran wrap with a sterile spore syringe using about 2 cc and cover hole with masking tape.
Incubate at 80 degrees Fahrenheit.
Once the mycelium has grown, shake to distribute the mycelium, and poke thru the saran wrap with a sterile syringe and suck up the white mycelium, cover the hole with tape and save for further use.