By Hippie3 (Hippie3) on Thursday, May 24, 2001 - 01:41 am: Edit

Myco-McGuyver
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This is how my monkey does his syringes every time...sterilizes shot glass in microwave(about 5min-hi) , he does this by putting his shot glass in a cup and filling the cup with water- then boiling it down so the shot glass can be removed with fingers once it's cooled ... he sometimes puts a bowl on the bottom to catch the splatter inside the microwave {Bowl-pyrex measuring cup[filled to 1cup capacity]-shot glass}boils some water, let's that cool... now the water that's left in the measuring cup can be used as syringe water- or if your syringes have been cooling because you just cleaned them (and their full of water) then you have plenty of water to make a syringe...
now ... what monkey does is pretty risky in some eyes in that he has this little home made innoculating loop... basicly what it is is a cheapo knife with the tip bent into a loop.. he then proceeds to scrape as many spores as he wants to into the shot glass, squirts out the contents (h2o)of the cooled syringe, sucks up and squirts out again to clean any of the spores that may have stuck to the sides.. sometimes he repeats this a couple of times if there aren't that many spores to be frivilous with.... i hope this helps out ... i think it would be easily applied to a jar or what ever glass container you have- that is , if you have a glass containter that is larger than it so you can sterilize it in the microwave

By Hippie3 (Hippie3) on Thursday, May 24, 2001 - 01:48 am: Edit

Bring some water to boil on the stove and let it boil about 10 minutes.
Suck the boiling water up to a fresh syringe.
If the syringe was used before, sterilize the allready filled syringe in a PC for 15 minutes.
Put syringe aside until cool.
Following tasks should be idealy prepared in front of a flow hood, but a reasonably cleaned bathroom will do:
Prepare the print, a shot glas and the alcohol lamp.
Rinse the clean shotglass with isopropanol or rubbing alcohol and let it evaporate a few seconds.
Bring the needle of the syringe to red glow above the alcohol lamp.
Squirt 1/2 ml of water out of the syringe to cool the needle and create some airspace in the syringe so later you can shake the spores.
Shake of the eventual drop on top of the needle off so the print won´t become wet.
Scrape some spores with the tip of the needle into the shotglas.
Add a small amount of water from the syringe, stir and suck it back into syringe.
Give the syringe a vigorous shake, let the spores rehydrate for a few hours and inoculate