By Admin (Admin) on Friday, June 08, 2001 - 05:08 pm: Edit

Sterile Tissue Cloning Procedure

1. Pour about 1/3 cup of 3% h2o2 into a jar.

2. Obtain a clean coffee grinder; fill with water to the brim of
the metal cup, insert a fresh and cleaned mushroom stem
(no cap) and proceed to grind for 10-15 seconds.
note: a blender can be substituted for a grinder.

3. Use a clean spoon to transfer some of the resulting mixture into
the h2o2 jar.

4. Let it fizzle for a couple minutes, then draw into a syringe.
optional: the syringe can be partially filled with sterile honey water beforehand to provide nutrients to sustain the tissue.

5. use immediately or refridgerate for a few days only.

Tips if Culture Contaminates
1. Extract a piece of healthy mycelium.
2. Cut mycelium into tiny pieces with razor blade.
3. Transfer pieces to a small pool of h2o2 and then suck into
a syringe.
4. Inoculate another culture jar with it.

plagarized and modified by hip from funkyballoon's post at the shroomery